Date of Original Version

5-1-2014

Type

Article

PubMed ID

24752080

Rights Management

© 2014 Nature America, Inc.

Abstract or Description

We introduce Sailfish, a computational method for quantifying the abundance of previously annotated RNA isoforms from RNA-seq data. Because Sailfish entirely avoids mapping reads, a time-consuming step in all current methods, it provides quantification estimates much faster than do existing approaches (typically 20 times faster) without loss of accuracy. By facilitating frequent reanalysis of data and reducing the need to optimize parameters, Sailfish exemplifies the potential of lightweight algorithms for efficiently processing sequencing reads.

DOI

10.1038/nbt.2862

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Published In

Nature biotechnology, 32, 5, 462-464.