Title

Genetically encoded pH sensor for tracking surface proteins through endocytosis.

Date of Original Version

5-14-2012

Type

Article

PubMed ID

22461279

Abstract or Description

Traffic cam: a tandem dye prepared from a FRET acceptor and a fluorogenic donor functions as a cell surface ratiometric pH indicator, which upon internalization serves to follow protein trafficking during endocytosis. This sensor was used to analyze agonist-dependent internalization of β(2)-adrenergic receptors. It was also used as a surrogate antigen to reveal direct surface-to-endosome antigen transfer between dendritic cells (not shown).

DOI

10.1002/anie.201108107

 

Published In

Angewandte Chemie (International ed. in English), 51, 20, 4838-4842.