Title

STED nanoscopy in living cells using Fluorogen Activating Proteins.

Date of Original Version

1-1-2009

Type

Article

PubMed ID

20976031

Abstract or Description

We demonstrate the effectiveness of a genetically encoded Malachite Green (MG) binding fluorogen activating protein (FAP) for live cell stimulated emission depletion nanoscopy (STED). Both extracellular and intracellular FAPs were tested in living cells using fluorogens with either membrane expressed FAP or as an intracellular FAP-actin fusion. Structures with FWHM of 110-122nm were observed. Depletion data however suggests a resolution of 70nm with the given instrument.

DOI

10.1021/bc900249e

 

Published In

Bioconjugate chemistry, 20, 10, 1843-1847.