Title

Synthesis of new fluorogenic cyanine dyes and incorporation into RNA fluoromodules.

Date of Original Version

4-17-2008

Type

Article

PubMed ID

18338898

Abstract or Description

A new fluorogenic cyanine dye was synthesized and found to have low fluorescence quantum yield in fluid solution and in the presence of double-stranded DNA but 80-fold enhanced fluorescence in viscous glycerol solution. An RNA aptamer selected for binding to the new dye exhibits K(d) = 87 nM and 60-fold fluorescence enhancement. The dye-aptamer pair is a fluoromodule that can be incorporated into fluorescent sensors and labels.

DOI

10.1021/ol702920e

 

Published In

Organic letters, 10, 8, 1561-1564.