Title

Cholera toxin B conjugated quantum dots for live cell labeling.

Date of Original Version

9-1-2007

Type

Article

PubMed ID

17663586

Abstract or Description

Cholera toxin subunit B (CTB)--quantum dot conjugates were developed for labeling mammalian cells. The conjugates were internalized by all tested cell lines into small vesicles dispersed throughout the cytoplasm, while commercially available polyarginine conjugates rapidly accumulated in large perinuclear endosomes. Although a large proportion of CTB conjugates eventually also accumulated in perinuclear endosomes, this accumulation required several days, and even then many CTB conjugated quantum dots remained in small vesicles dispersed throughout the cytoplasm. Thus CTB conjugates are a practical alternative to polyarginine conjugates for the general labeling of mammalian cells.

DOI

10.1021/nl0709930

 

Published In

Nano letters, 7, 9, 2618-2626.