Title

Mini-blaster-mediated targeted gene disruption and marker complementation in Candida albicans.

Date of Original Version

1-1-2012

Type

Article

PubMed ID

22328365

Abstract or Description

Several gene disruption strategies have been described in Candida albicans to create homozygous mutants. We describe here a recyclable mini-blaster cassette containing C. albicans URA3 gene and 200-bp flanking repeats that is useful for disruption of C. albicans genes. The cassette can be used to create unmarked homozygous mutants which can be complemented at the HIS1 gene locus. This strategy of creating gene disruptions and subsequent complementation can be used to study gene function.

DOI

10.1007/978-1-61779-539-8_2

 

Published In

Methods in molecular biology, 845, 19-39.