Title

GM130 and GRASP65-dependent lateral cisternal fusion allows uniform Golgi-enzyme distribution.

Date of Original Version

3-1-2006

Type

Article

PubMed ID

16489344

Abstract or Description

The mammalian Golgi apparatus exists as stacks of cisternae that are laterally linked to form a continuous membrane ribbon, but neither the molecular requirements for, nor the purpose of, Golgi ribbon formation are known. Here, we demonstrate that ribbon formation is mediated by specific membrane-fusion events that occur during Golgi assembly, and require the Golgi proteins GM130 and GRASP65. Furthermore, these GM130 and GRASP65-dependent lateral cisternal-fusion reactions are necessary to achieve uniform distribution of enzymes in the Golgi ribbon. The membrane continuity created by ribbon formation facilitates optimal processing conditions in the biosynthetic pathway.

DOI

10.1038/ncb1366

 

Published In

Nature cell biology, 8, 3, 238-248.