Title

Internet-based image analysis quantifies contractile behavior of individual fibroblasts inside model tissue.

Date of Original Version

4-1-2003

Type

Article

PubMed ID

12668480

Abstract or Description

In a cell-populated collagen gel, intrinsic fiber structure visible in differential interference contrast images can provide markers for an in situ strain gauge to quantify cell-gel mechanics, while optical sections of fluorescent protein distribution capture cytoskeletal kinematics. Mechanics quantification can be derived automatically from timelapse differential interference contrast images using a Deformation Quantification and Analysis software package accessible online at http://dqa.web.cmu.edu. In our studies, fibroblast contractile machinery was observed to function entirely within pseudopods, while GFP-alpha-actinin concentrated in pseudopod tips and cortex. Complex strain patterns around individual cells showed instances of both elastic and inelastic strain transmission, suggesting a role in observed long-range alignment of cells.

DOI

10.1016/S0006-3495(03)75077-2

 

Published In

Biophysical journal, 84, 4, 2715-2727.