Title

Fluorogen-activating proteins as biosensors of cell-surface proteins in living cells.

Date of Original Version

8-1-2010

Type

Article

PubMed ID

20653017

Abstract or Description

This study explores the general utility of a new class of biosensor that allows one to selectively visualize molecules of a chosen membrane protein that are at the cell surface. These biosensors make use of recently described bipartite fluoromodules comprised of a fluorogen-activating protein (FAP) and a small molecule (fluorogen) whose fluorescence increases dramatically when noncovalently bound by the FAP (Szent-Gyorgyi et al., Nat Biotechnol 2010;00:000-000).

DOI

10.1002/cyto.a.20925

 

Published In

Cytometry. Part A, 77, 8, 776-782.