Title

Expression of kinesin in Escherichia coli.

Date of Original Version

1-1-2001

Type

Book Chapter

PubMed ID

11217613

Abstract or Description

Kinesin can generally be expressed to high levels in Escherichia coli and this provides an excellent means for obtaining significant amounts of purified constructs. For detailed work, it is essential to have a native construct uncompromised by extraneous tags, and this can readily be obtained. Seerefs. 1–3 for examples of vector construction using the T7 RNA polymerase system.

DOI

10.1385/1-59259-069-1:43

 

Published In

Methods in molecular biology, 164, 43-48.